Journal: Progress in Biomaterials
Article Title: Hydrogel scaffolds with elasticity-mimicking embryonic substrates promote cardiac cellular network formation
doi: 10.1007/s40204-020-00137-0
Figure Lengend Snippet: Shown in a are cells cultured within the 3D gels and on 2D Matrigel controls. The cells were pre-stained with PKH26 (red) to reveal network formation in these cultures. Also, the magnified insets depict the elongated cell morphology within the embryonic gels, which became gradually rounded as the gels were progressively stiffened from the physiologic to the fibrotic gels. Shown in b is the average cellular aspect ratio (width/height) for cells cultured within the gels. The aspect ratio is maximum in the case of cells cultured within the embryonic gels and is close to the value of 1 (implying rounded cells) as the gels were stiffened
Article Snippet: To confirm the viability of cells, post-encapsulation in gels after crosslinking, the CM were labeled with PKH26 red fluorescent membrane staining dye (Sigma), as done before (Anil Kumar et al. 2019a , b ).
Techniques: Cell Culture, Staining